Electrophysiology: Micro Electrode Array (MEA) system, solution changer, puller, micromanipulator

 


Rapid Solution Changers support


RSC-200 (and RSC-160) Software upgrade

Click here to download the current version. Then create an empty directory, unzip the downloaded file in this directory and run [setup.exe].

Version history

  • RSC V1.04 (June 12, 2003) :
    • Modification in the management of the GPIB library to improve the compatibility of the software with all the versions of Windows.
    • Graphic interface : minor bug correction.
  • RSC V1.03 (December 14, 2001) :
    • The software is compatible with Win9x, Me, NT, 2000 and XP.
    • Selection of the RSC model (RSC-160 or RSC-200) into the menu : " Setup ® RSC model… ".
    • Graphic interface : minor bug correction.

Electrovalve array (EVH-9) starting instructions

Setting up

First of all, the reservoir syringes, inserted in the syringe rack has to be fixed on any type of the support which permits a relatively easy adjustment of the height. The height of the rack determines a flow rate of the solutions. In general a syringe rack holder (a vertical metal bar, diameter of 15mm) is used. On the same rack the EVH-9 module can be fixed, below the syringe rack.

Tubing

The EVH-9 electrovalve array can work with tubing of different types, like silicone, or C-Flex. The outside diameter should not exceed 2.5mm. Also the wall rigidity has to be taken into account, a tubing to hard to be squeezed by the valve spring, may result the valve leakage. Our preference is C-Flex tubing because of its superior impermeability to gasses (ID: 0.5mm, OD: 2.1mm ; COLE PARMER ref : 6424-59 ), normally delivered with the system.

  1. cut the tubing to the required length
  2. insert the luer lock (a tiny, plastic connector to be fit on the tip of the syringe, delivered with the system) on one end of the tubing and connect to the syringe tip. Make sure that the tubing is inserted straight and the flow is not blocked.
  3. introduce the tubing into the valve. This step of installation has to be done carefully to assure a good functioning of the system. The following pictures show the procedure :


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ressing the central part of the valve opens the valve

 


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Top view : The upper valve is in open state, the space where the tubing should be inserted is well seen. The valve below is in closed state.


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With the valve opened with one finger, insert the tubing, holding it vertical and slightly stretched. When the tubing is inserted in the valve's groove, make sure it is well vertical and in the groove axis. Do not stretch the tubing excessively.

Make sure that the tubing is not stretched between the syringe and the valve, what may block the flow or cause the leakage.

Flow test

Having installed all tubing in the valves, fill the syringes with water and check all valves one by one. Open the valve by control unit or by hand and see if the water is flowing correctly, and stops when the valve is closed. In case of any problem check : - the tubing is not blocked on the level of luer lock, - the tubing is not under tension between the syringe and the valve, - the tubing is well in the valve groove - the tubing is not blocked inside (use a syringe piston to push the solution)

Rapid Solution Changer improvements

New head for RSC-100, RSC-160 and RSC-200

We have developed a new head - the "straight head" - which is the head by default for the RSC-160 and RSC-200 supplied from January 1998. The straight head was designed to carry standard capillary glass, OD 1.00mm.

This head has significant advantages over the others:

  • Capillaries can protrude, giving better approach to a cell.
  • The number of capillaries installed is up to the user.
  • Capillaries take minimum space in the cuvette.
  • Easy exchange of capillaries.
  • Easy attachment to the flexible tubing

You can upgrade easily your RSC-100, RSC-160 or RSC-200 with this new head.

Note: Glass with outside diameter different from 1.00 mm can be used with the RSC-160 or RSC-200 and the V1.02 version firmware (or higher).

For more information about this head, click here.

Reference Description
025-00/17 STRAIGHT ROTATING HEAD

Better shielding and new motor for RSC-160 & RSC-200

Since January 1998, the shielding of the stepping motor is connected to the RSC-160 & RSC-200 through a pin of the connector rather than using the connector itself. We have indeed found some connectors with loose connection. If you have encountered such a problem, we recommend that you use a wire to set a good contact between the stepping motor and the RSC-160 & RSC-200.

Since January 1998, the RSC-160 & the RSC-200 are supplied with a new and compatible stepping motor. This motor has important advantages over the previous version:

  • Much lighter: 220 g as compared to 460 g
  • Much smaller. See drawing.
  • Direct mounting (no adaptor required - better mechanical link) on the following micromanipulators: UM-3C (with rotation mechanism) (You), M-152 (Narishige), MM-33 (Marzhauser)
  • Power and working temperature divided by 2 (except at 2ms per tube).

RSC-160 & RSC-200 Stepping motor I (old model)

 

RSC-160 & RSC-200 Stepping motor II (new model)

 


RSC-160 & RSC-200 Stepping motor II mounted on UM-3C micromanipulator

RSC-160 & RSC-200 Stepping motor II mounted on M-152 micromanipulator

RSC-160 & RSC-200 Stepping motor II mounted on MM-33 micromanipulator

As the new stepping motor is compatible with the previous version, you can upgrade your system easily. A shielding wire is supplied for connection to RSC-160 & RSC-200 delivered before January 1998.

Reference Description
025-00/20 RSC-160 & RSC-200 Stepping motor II

New firmware for RSC-160 & RSC-200

To get the version number of your RSC-160 & RSC-200 firmware, keep the "STATUS" button pressed on the front panel.

The firmware upgrade consists of the exchange of 2 x EPROM chips of the latest version (operation not warrantied if not done in factory).

Reference Description
025-00/19 RSC-200 firmware upgrade
025-00/23 RSC-160 firmware upgrade

Version history

  • RSC-200 & RSC-160 Firmware V1.05a (March 1, 2000):
    • The RSC-160 now accept up to 36 tubes (number of electro valve is still limited to 9)
  • RSC-200 & RSC-160 Firmware V1.05 :
    • The firmware is modified for the RSC-160 (same source for both RSC-160 & RSC-200 but different firmware).
  • RSC-200 - Firmware V1.04 :
    • Small bug in the number of micro step is now corrected
  • RSC-200 - Firmware V1.03 :
    • In the ANALOG CONTROL-IN mode (a signal sent to the analog input can drive the rotating head and the valve opening) a delay can now be added between valve opening and tube movement: When the analog signal is detected, the corresponding valve is opened. After a programmable delay [0..200ms], the tube is moved to its corresponding position and the previous valve is closed.
  • RSC-200 - Firmware V1.02 :
    • Fixed step number per tube is now replaced by programmable step number per tube.
  • RSC-200 - Firmware V1.01 :
    • First commercial release.

New manual for the MSC-200 (May 2003)

Click here for download

Preparing the straight head for an experiment

Mounting the capillaries

1) Check the capillaries with a calliper for their OD variation
2) Place the joints close to the metal pins. Insert the capillaries under the two joints on the head. Pushing the joint on both sides, with your fingers, upwards, will create a small gap to insert the capillaries. Slip all capillaries under first joint and then go to the second one.
3) Now the capillaries need to be brought together. Instead, however of pushing the fragile glass, turn the joints.

Alignment of capillaries

Push capillaries out...

...and then use a straight and clean surface to align them.

Check their parallel position and put the scotch or parafilm around the head and capillaries (between joints) to immobilise glass tubes in their position.

Mounting the head on the motor

Insert entirely the motor axis. Tighten the black screw clockwise (with the tip in front of you), using a fork wrench. To keep the head axis on place when screwing, the hole for grounding can be useful.

Head grounding

Use the thin cable and the provided pin to connect the head to the closest grounding.

High Flow-Rate set-up

If a high flow rate is required, you can connect directly the glass capillaries to the large flexible tubing (the polyethylene tubing is then not used). In this case, however, the rear glass end must be bent to give necessary space for tubing: place the capillary just above a flame and wait until the capillary bends downwards under its own weight (30° angle and 1cm length approximately).

Rotating head diagrams


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Straight rotating head


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4-holes rotating head


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6-holes rotating head


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9-holes rotating head


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18-holes rotating head

 
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